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(A) B cells: CD20 (red) and <t>CD79b</t> (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.
Mouse Anti Human Cd79b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human cd79b - by Bioz Stars, 2026-02
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human respiratory syncytial virus nr-28528  (BEI Resources)
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(A) B cells: CD20 (red) and <t>CD79b</t> (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.
Human Respiratory Syncytial Virus Nr 28528, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human respiratory syncytial virus nr-28528 - by Bioz Stars, 2026-02
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atcc 28528  (ATCC)
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(A) B cells: CD20 (red) and <t>CD79b</t> (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.
Atcc 28528, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc 28528 - by Bioz Stars, 2026-02
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us cr ip t ac ce pt ed  (ATCC)
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(A) B cells: CD20 (red) and <t>CD79b</t> (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.
Us Cr Ip T Ac Ce Pt Ed, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti igβ  (Novus Biologicals)
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(A) B cells: CD20 (red) and <t>CD79b</t> (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.
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anti igβ - by Bioz Stars, 2026-02
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goat anti-iba1 sc-28528  (Santa Cruz Biotechnology)
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(A) B cells: CD20 (red) and <t>CD79b</t> (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.
Goat Anti Iba1 Sc 28528, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) B cells: CD20 (red) and CD79b (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.

Journal: The Journal of Clinical Investigation

Article Title: B cells, antibody-secreting cells, and virus-specific antibodies respond to herpes simplex virus 2 reactivation in skin

doi: 10.1172/JCI142088

Figure Lengend Snippet: (A) B cells: CD20 (red) and CD79b (green) in serial genital skin biopsy samples taken during and after HSV-2 lesion from a single participant (results shown for 1 of 10 patients). No B cells were detected in the control sample. (B) T cells: CD4 (green) and CD8 (red) in serial specimens taken during and after HSV-2 reactivation (result shown for 1 of 8 patients). Scale bars: 100 μm. (C) Densely clustered CD4+CD8+ T cells (left) in this participant were accompanied by CD20+CD79b+ B cells (right); scale bars: 50 μm. (D) CD27 (green) and CD20 (red) in a lesion sample with no evidence of costaining (result shown for 1 of 6 patients); scale bars: 50 μm. (E) In a healing-skin specimen, B cells express CD20 (green) and IgD (red) (result shown for 1 of 5 patients). Arrows indicate sites of coexpression. Inset shows red and green channels separately. Scale bars: 50 μm. (F) IgG-producing cells are loaded with IgG RNA by FISH. IgG mRNA (green) in a healing specimen of HSV-2 reactivation (result shown for 1 of 9 patients). Original magnification, 40× by oil immersion. Scale bar: 25 μm. (G) IgG+ cells by FISH over time, showing distribution within the upper dermis and lymphocyte clusters. In this participant, the control specimen (from the arm) had no IgG+ cells. Dashed line indicates the DEJ. Images obtained at 20×; scale bars: 250 μm. Brightness in G was increased for consistency. wph, weeks after healing.

Article Snippet: To enumerate the relative density of B cells, T cells, ASCs, blood vessels, and/or antibody in tissue biopsy sections, the following primary antibodies were used; rabbit anti–human CD20 (1:100; Abcam ab78237), mouse anti-human CD79b (1:100; Novus Biologicals NBP1-28528), mouse anti-human IgG (1:100; eBioscience 05-4200), sheep anti–human vWF (FITC-conjugated, 1:5000; Abcam ab8822), mouse anti–human CD27 (1:100; BioLegend 356403), mouse anti–human CD20 (1:100; eBioScience 14-0202-82), mouse anti-human IgD (af647-conjugated, 1:50 or 1:100; BioLegend 348227), rabbit anti–HSV-2 (1:1000; Dako B0116), mouse anti-CD4 (1:1000; BioLegend 300502), and mouse anti-CD8 (af647-conjugated, 1:100; BD Pharmingen 557708).

Techniques: Control